The Tang Dynasty’s Female Forensic Doctor - Chapter 56

[Chapter 59 Penicillin]

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Returning to the courtyard, Ran Yan saw Xing Niangxun sitting under the porch cutting fabrics, looking unhappy, and sighed for a while.

When Xing Niang saw Ran Yan coming back, she hurriedly responded, took Mi Li, looked at Ran Yan’s face, and said distressedly: “Is your mother tired? I will go back to the main house in a few days, and the person who seeks your medical treatment guesses It

will be much less.”

“Well, Madam Xing, help me make the bath soup, I want to take a bath.” Ran Yan said.

Xing Niang said with a smile: “It’s already ready, it’s time to take a bath to get rid of the sickness.”

Ran Yan’s body has not recovered for a long time, but she was already weak. On this day, two patients were diagnosed and two corpses were dissected. .

Ran Yan was a little drowsy after soaking in the warm water.

“Mother, Xing Niang has sent slaves to wait for you to take a bath.” A crisp voice sounded outside.

Ran Yan was not used to someone watching her take a bath, but this time she was really tired, so she could ask the maid to help wash the damn long hair, and said, “Come in.” The person who came

was Xiaoman who was dressing Ran Yan in the morning .

He was wearing a small bathtub with a wooden cover, and there was a faint heat in the gap, and a floral fragrance escaped in the air.

“What is it?” Ran Yan asked.

Xiaoman knew that Ran Yan was not as scary as she looked, so she smiled lightly and opened the small dimples on her cheeks, “Seeing that the honeysuckle in the courtyard is blooming well, this servant picked some to give her mother a bath, and use honeysuckle in summer. It’s good to take a bath!”

Ran Yan nodded, but thought to herself, Xiao Song really guessed right, Fan Chun was probably drowned in the bathtub, but… Whose bathtub did she drown in?

Seeing that Ran Yan didn’t speak, Xiaoman boldly stepped forward and said, “Mother, will this servant help you wash your hair?”

“Okay.” Ran Yan replied.

Xiaoman pulled a high stool from the corner of the wall, put it next to the tub, and put the small tub on top.

Ran Yan slid water on her body and watched Xiaoman’s movements, her pupils shrank suddenly – a high stool and a wooden barrel.

The high stool in the bathroom is a bit rough, but it is very similar to the stool that fell to the ground on the day Wan Lu was hanged. People in the Tang Dynasty still sat on their feet. The only seat of the chair and stool is the Hu bed, and the Hu bed is far from that high.

Also, this bucket lid…

“Xiaoman, stand across from me.” Ran Yan said slowly.

Xiaoman was pouring the petal bath soup into the tub. Hearing that Ranyan remembered her name, he answered happily, and happily placed the remaining half-pail of water on the high stool beside the tub, and stood according to the words. to the opposite side.

Ran Yan stretched out her hand from the tub, touched the small wooden tub, and poured all the water in it into the tub.

Then I started to study the barrel and the lid of the wooden barrel. From time to time, I gestured at Xiaoman’s head with these two things, and Xiaoman’s heart trembled, for fear that she would really throw something on his head. .

This wooden barrel has a wide mouth and a narrow bottom, but it is very heavy. Whether it is the bottom or the mouth, it will not just be swollen. .

The sizes of the buckets are different, and the arcs are different. The murderer just slammed the bucket lid onto Fanchun’s face in a hurry, and immediately found out that she was not dead, nor fainted…

Ran Yan stood up from the bucket with a clatter, and reached out her hand. Hold Xiaoman’s neck.

Xiaoman stared dumbfounded at Ran Yan’s bumpy body, her suet jade-like skin was dazzling with water, standing in the mist, she was fascinated.

She was fascinated, but Ran Yan grabbed her neck abruptly, pushing down the edge of the tub abruptly, her face almost touching the surface of the water, she hurriedly shouted: “Mother, spare your life, mother!”

Ran Yan turned back. God, found himself standing naked in the tub, coughed awkwardly, sat back in the water silently, and explained to the frightened Xiaoman, “I… just thought of something.”

Hearing such an explanation, Xiao Manke didn’t feel relieved at all, thinking of something, and then gestured at her head with the bucket lid, and then tried to press her into the water?

But… the mother’s body is really worth seeing!

Xiaoman’s watery eyes glanced at the looming body in the water, and whispered: “Mother, this servant will wash your hair for you.”

Ran Yan nodded, and now she has figured out some things, so she will not bother. , and let Xiaoman help her comb her black hair that was longer than her waist and hips.

After finally taking a bath, Ran Yan immediately went back to the room to grind, and drew the shape of the tub and the high stool, and then wrote a letter and handed it to Yang Yong, who was guarding the village, and asked him to immediately. Send someone to Liu Shishi.

After all this was done, Ran Yan was really relaxed. After dinner, she went to accompany Wan Lu for a while, and then went back to the bedroom to rest.

Xu Shi was terribly tired. Although there were many fragmentary scenes in this night’s dream, they were never connected together. It was the best sleep in the past six or seven years.

The next few days were cloudy and rainy, and it was very inconvenient to travel. Therefore, people who asked Ran Yan to diagnose the disease all sent letters saying that they did not dare to bother her to visit the door in person, and would bring the patient to the door on the agreed date.

So these days, Ran Yan has been free. After seeing patients every day, he went to Wu Xiuhe to listen to his medical lectures. Since Ran Yan made that meal, Wu Xiuhe is obviously more attentive and more diligent than before. , From time to time, he signaled to her that she should cook a meal to reward him for his hard work.

Unconsciously, five days passed, and the Penicillium in the culture medium was almost ready to extract penicillin.

Ran Yan poured the liquid in ten petri dishes into a special wooden barrel prepared in advance, then poured vegetable oil into the barrel and mixed it.

Penicillium water contains fat-soluble substances, and oil can dissolve and separate such fat-soluble substances.

After mixing well, let it sit and wait.

In the end, the material in the barrel will be separated into layers. The first layer is the fat-soluble substance that is dissolved in oil, and the second layer is the insoluble substance that is insoluble in water and oil. Because penicillin is a water-soluble substance, so The bottom water contains penicillin.

The bottom of the specially-made wooden barrel is like a wine barrel. There is a wooden stopper. When the wooden stopper is pulled out, the water at the bottom slowly flows out. Ran Yan put it into a porcelain bowl sterilized with wine and distilled water.

After filtering some of the large impurities, pour it into a jar, which contains boiled and sterilized crushed carbon. After stirring, the penicillin will be absorbed by the carbon.

Xing Niang, who was helping by the side, looked blankly at Ran Yan dumping a bunch of bottles and jars. She didn’t understand if a bucket was filled with mold and left for seven days of water, would it make any difference after oiling and carbonization?

In fact, these steps in today’s operation are not difficult at all. The difficulty is the preparation of these materials. Just the wine and distilled water used for disinfection make Ran Yan busy for a long time every day, especially the collection of distilled water, although It’s not difficult, but the amount is small, it takes a lot of time to collect a bottle, and when it is sterilized, half of the bottle is poured out at once, which is just pouring Ran Yan’s blood and sweat.

There is also sodium carbonate required for filtration, commonly known as alkali and soda, which is made from salt, sulfuric acid, coal, and limestone. Fortunately, Ran Yan has been nurtured by her father for several years. Can only stare.

After Ran Yan stirred the carbon and water-soluble substances evenly, it was purified again and added: distilled water, baking soda water, weak acid water (vinegar + water).

Add distilled water for the first time and filter, the water poured out is not penicillin solution and can be discarded.

The weak acid water was added for the second time, and the filtered water was still thrown away.

The first step is to add baking soda water, and the filtered solution is the real penicillin aqueous solution.

Ran Yan looked at the four bottles containing the penicillin aqueous solution, she was always calm, her eyes were slightly red-it was not easy!

These days, just getting distilled water makes her back sore.

Originally, when she thought about seeing the syphilis on Zixu’s body, Ran Yan didn’t think much, because as a psychological modern person, a modern doctor, subconsciously, he felt that the extraction procedure of penicillin was a relatively simple thing, but only after he did it would he know it. , There was no soda or distilled water in the Tang Dynasty, the fine carbon had to be ground slowly, and the medium solution had to be boiled with taro and rice…

How could such a difficult disease appear in the Tang Dynasty!

Ran Yan sighed in her heart that if she could change it, she would rather dissect two corpses every day than make penicillin.

“Mother, this is the fairy water?” Xing Niang asked in a low voice.

Ran Yan paused for a moment, then remembered that she had said that someone in her dream was giving advice, so she had to nod her head.

Ran Yan poured two drops into another petri dish, which contained Staphylococcus aureus. This strain is relatively easy to obtain. Ran Yan found a little a few days ago and put it in the culture medium to promote its growth. Today there are It’s full.

Staphylococcus aureus gradually disappeared with the dripping of the penicillin aqueous solution, and the place where it was dripped was quickly vacated.

The verification was useful, and Ran Yan breathed a sigh of relief. If it took a lot of hard work and the last step was still invalid, then she would really cry, but fortunately, God was not mean to her.

Ran Yan carefully put away these hard-won bottles of penicillin, and finally put down the big stone in her heart. She felt quite relaxed, and decided to cook a meal for the cheap master.

Wu Xiuhe stretched his neck and waited for a long time, UU reading www.sonicmtl.com Ran Yan just made the first dish, Ran Yunsheng came, so angry that the old man blew his eyes and stared at him. , disintegrates in an instant, rudely, and does anything.

In desperation, Ran Yan had to cook his favorite Dongpo pork, one meat and one vegetarian dish, to comfort his broken heart.

Ran Yunsheng smiled brightly, “Ayan, your master is really interesting.” The corners of

Ran Yan’s lips curved slightly, and instead of answering the topic, she asked, “Are you done?”

“Have you forgotten?” Ran Yunsheng paused and said angrily, “Today is the Qixi Festival! How can you, as a daughter’s family, forget everything!”

“Oh.” Ran Yan remembered that Xing Niang started early in the morning Busy chattering.

“Come on, go and freshen up, we’re going to Pingjianghe tonight, and the uncle asked me to take you there too!” Ran Yunsheng urged hurriedly.

…

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[End of this Chapter]